sc 365322  (Santa Cruz Biotechnology)

Journal: Molecular Medicine Reports

Article Title: Effects of natural killer cell‑conditioned medium on UVB‑induced photoaging in human keratinocytes and a human reconstructed skin model

doi: 10.3892/mmr.2025.13488

Figure Lengend Snippet: Primer sequences used for quantification of gene expression.

Article Snippet: Anti-hyaluronan synthase3 , 1:5,000 , sc-365322 , Santa Cruz Biotechnology, Inc..

Techniques: Expressing, Sequencing

Journal: Molecular Medicine Reports

Article Title: Effects of natural killer cell‑conditioned medium on UVB‑induced photoaging in human keratinocytes and a human reconstructed skin model

doi: 10.3892/mmr.2025.13488

Figure Lengend Snippet: Antibodies used for western blot analysis.

Article Snippet: Anti-hyaluronan synthase3 , 1:5,000 , sc-365322 , Santa Cruz Biotechnology, Inc..

Techniques: Western Blot

Journal: Proteoglycan Research

Article Title: p53 and ΔNp63 Transcriptional Programs in Coordination With TGF‐β1 and RAS Activation Regulate HAS2 and HAS3 in Epithelial Cells

doi: 10.1002/pgr2.70015

Figure Lengend Snippet: FIGURE 1 | Contrasting effects of endogenous mutant p53 and p63 on HAS2 and HAS3 expression in HaCaT cells. (A) Microarray Suite software 5.0 (Affymetrix) was used to calculate the average difference for each gene probe set, depicted as the gene expression intensity value; unpublished data from Ref. 18. (B) Antagonistic relationship between mutant p53 and p63 expression in HaCaT cells. Gene expression in HaCaT cells was determined by qRT‐PCR and normalized by GAPDH mRNA expression. (C) p63 positively regulates HAS2 and HAS3 mRNA expression in HaCaT cells. Levels of gene expression were determined by qRT‐PCR and normalized by GAPDH mRNA expression. Data are means ± SD; n = 2 experiments; **p < 0.01, ***p < 0.001 by Students t‐test.

Article Snippet: The membranes were incubated at 4°C overnight with antibodies against HAS2 (Santa Cruz Biotechnology, sc‐ 514737), HAS3 (Santa Cruz Biotechnology, sc‐34204), tubulin (Sigma Aldrich, T5168), p53 (Santa Cruz Biotechnology, sc‐ 6243), or p63 (Abcam, ab124762), followed by incubation with horseradish peroxidase‐conjugated secondary antibodies for 1 h at room temperature, and development by chemiluminescence (Millipore, Burlington, MA, USA).

Techniques: Mutagenesis, Expressing, Microarray, Software, Gene Expression, Quantitative RT-PCR

Journal: Proteoglycan Research

Article Title: p53 and ΔNp63 Transcriptional Programs in Coordination With TGF‐β1 and RAS Activation Regulate HAS2 and HAS3 in Epithelial Cells

doi: 10.1002/pgr2.70015

Figure Lengend Snippet: FIGURE 2 | RAS activation regulates TGF‐β1‐mediated HAS2 ex- pression, but not HAS3 expression. (A, B) Depiction of the binding strength of p63 on HAS2 (A) and HAS3 (B) genomic loci without or with RAS activation and TGF‐β1 stimulation; unpublished data from Ref. 18. (C) qRT‐PCR validated HAS2 mRNA expression in HaCaT cells under active RAS and TGF‐β1 stimulation. HAS2 mRNA was normal- ized by GAPDH mRNA expression. Data are means ± SD; n = 2 ex- periments; **p < 0.01, ***p < 0.001 by Students t‐test.

Article Snippet: The membranes were incubated at 4°C overnight with antibodies against HAS2 (Santa Cruz Biotechnology, sc‐ 514737), HAS3 (Santa Cruz Biotechnology, sc‐34204), tubulin (Sigma Aldrich, T5168), p53 (Santa Cruz Biotechnology, sc‐ 6243), or p63 (Abcam, ab124762), followed by incubation with horseradish peroxidase‐conjugated secondary antibodies for 1 h at room temperature, and development by chemiluminescence (Millipore, Burlington, MA, USA).

Techniques: Activation Assay, Expressing, Binding Assay, Quantitative RT-PCR

Journal: Proteoglycan Research

Article Title: p53 and ΔNp63 Transcriptional Programs in Coordination With TGF‐β1 and RAS Activation Regulate HAS2 and HAS3 in Epithelial Cells

doi: 10.1002/pgr2.70015

Figure Lengend Snippet: FIGURE 3 | Opposite effect of TGF‐β1 stimulation on HAS2 and HAS3 mRNA expression after mutant p53 knockdown. (A–C) HaCaT cells were stimulated with TGF‐β1 (5 ng/mL for 24 h), after which mRNA was prepared and subjected to qRT‐PCR for HAS2 (A), HAS3 (B) and p53 (C). (D) Hyaluronan secreted to the conditioned media of HaCaT cells in which p53 was knocked down, or not, was determined after non‐stimulation or stimulation with TGF‐β1 or 10% FBS. (E) p53‐null H1299 cells were transfected with p53 WT, as well as with p53 mutants (R175H and R273H). Data are means ± SD; n = 3 experiments; *p < 0.05, **p < 0.01, ***p < 0.001 by Students t‐test.

Article Snippet: The membranes were incubated at 4°C overnight with antibodies against HAS2 (Santa Cruz Biotechnology, sc‐ 514737), HAS3 (Santa Cruz Biotechnology, sc‐34204), tubulin (Sigma Aldrich, T5168), p53 (Santa Cruz Biotechnology, sc‐ 6243), or p63 (Abcam, ab124762), followed by incubation with horseradish peroxidase‐conjugated secondary antibodies for 1 h at room temperature, and development by chemiluminescence (Millipore, Burlington, MA, USA).

Techniques: Expressing, Mutagenesis, Knockdown, Quantitative RT-PCR, Transfection

Journal: Proteoglycan Research

Article Title: p53 and ΔNp63 Transcriptional Programs in Coordination With TGF‐β1 and RAS Activation Regulate HAS2 and HAS3 in Epithelial Cells

doi: 10.1002/pgr2.70015

Figure Lengend Snippet: FIGURE 4 | Activation of RAS affects hyaluronan synthesis in the MCF10 series of breast epithelial cells. (A) mRNA expression of HAS2, HAS3 and CD44 was determined by qRT‐PCR in MCF10A. M1‐4 cells stimulated or not with TGF‐β1 (5 ng/mL for 24 h). (B) Hyaluronan concentrations in the conditioned media were determined in the same conditions. Data are means ± SD; n = 3 experiments; *p < 0.05, **p < 0.01, ***p < 0.001 by Students t‐test.

Article Snippet: The membranes were incubated at 4°C overnight with antibodies against HAS2 (Santa Cruz Biotechnology, sc‐ 514737), HAS3 (Santa Cruz Biotechnology, sc‐34204), tubulin (Sigma Aldrich, T5168), p53 (Santa Cruz Biotechnology, sc‐ 6243), or p63 (Abcam, ab124762), followed by incubation with horseradish peroxidase‐conjugated secondary antibodies for 1 h at room temperature, and development by chemiluminescence (Millipore, Burlington, MA, USA).

Techniques: Activation Assay, Expressing, Quantitative RT-PCR

Journal: Cells

Article Title: 4-Methylumebelliferone Enhances Radiosensitizing Effects of Radioresistant Oral Squamous Cell Carcinoma Cells via Hyaluronan Synthase 3 Suppression

doi: 10.3390/cells11233780

Figure Lengend Snippet: Effects of St -Hyal treatment or HAS3 knockdown on RR cells. ( A ) Protein expression analysis of siRNA-HAS3 transfection via Western blotting. Representative images of immunoblots are shown. Actin was used as a loading control, and the relative values of the HAS3/actin ratio are presented. For the HAS3 proteins, both bands were quantified together. ( B , C ) Logarithmic surviving fraction of each cell line treated with ( B ) 100 TRU/mL St -Hyal and IR or ( C ) HAS3 knockdown and IR. ( D ) Representative histograms of each cell line treated with 100 TRU/mL St -Hyal or with HAS3 knockdown. ( E ) Relative MFI of CD44 of each cell line treated with 100 TRU/mL St -Hyal or with HAS3 knockdown. ( F ) Relative SOD production levels of each cell line treated with HAS3 knockdown and IR. The SOD production levels of IR alone, HAS3 knockdown, and combined treatment groups were standardized based on the SOD level of the control group; * and ** indicate p < 0.05 and p < 0.01 vs. control, respectively; # and ## indicate p < 0.05 and 0.01 vs. 2 Gy, respectively.

Article Snippet: Small interfering RNA (siRNA) against HAS3 (sc-45295), the corresponding scrambled control siRNA (sc-37007), and anti-HAS3 (sc-365322) monoclonal primary antibodies were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA).

Techniques: Knockdown, Expressing, Transfection, Western Blot, Control